Copper and cadmium was coupled to carrier proteins such as keyhole limpet hemocyanin (KLH) and bovine serum albumin (BSA) via bifunctional chelator. Heavy metal-chelator-KLH had taken as immunizing antigen and metal-chelator-BSA as detection antigen.
将重金属铜离子、镉离子通过双功能螯合剂与血蓝蛋白偶联制备免疫抗原,与牛血清白蛋白偶联制备检测抗原。
参考来源 - 重金属铜、镉快速免疫检测技术研究The synthesis of bifunctional chelator was the key of time-resolved fluorescence immunoassay. The so-called bifunctional chelator could be coordinated with rare earth ion; on the other hand, it could also contain appropriate functional to be covalently attached to biomolecules.
时间分辨荧光免疫分析法中重要的是双功能螯合剂的合成,所谓的双功能其一要实现与稀土离子配位,其二,在不影响荧光性能条件下能实现与生物分子以共价键相连。
参考来源 - 时间分辨荧光免疫分析双功能类三吡啶型螯合剂中间体的合成与表征·2,447,543篇论文数据，部分数据来源于NoteExpress
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